normal rat renal tubular epithelial cell line nrk52e  (ATCC)

Journal: Scientific Reports

Article Title: Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell Model

doi: 10.1038/s41598-019-39533-3

Figure Lengend Snippet: MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 10 4 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. ( a ) NRK52E + testosterone (1~10 3 nM), ( b ) NRK52E + bicalutamide (3.75~60 μM), ( c ) RMC + testosterone (1~10 3 nM), ( d ) RMC + R1881 (1~10 3 nM), and ( e ) RMC + bicalutamide, and ( f ) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate

Article Snippet: NRK52E, a rat normal renal proximal tubular epithelial cell line; and RMC, the rat mesangial cell line, were provided by the Bioresource Collection & Research Center (BCRC; Hsinchu, Taiwan).

Techniques: MTT Assay, Incubation